olig2 polyclonal rabbit anti human antibody  (Proteintech)

Journal:

Article Title: Interferon-triggered transcriptional cascades in the oligodendroglial lineage: a comparison of induction of MHC class II antigen between oligodendroglial progenitor cells and mature oligodendrocytes

doi: 10.1016/j.jneuroim.2009.04.021

Figure Lengend Snippet: Surface expression of major histocompatibility complex on cells of rat oligodendroglial lineage in response to IFNG or IFNB. Surface immunoreactivities for RT1A and RT1D in OPC (A) and MO (B) were examined by immunocytochemistry at 48 h after treatment with IFNG, IFNB or medium alone (control). RT1A (class I, green) was absent in control cultures, and more strongly induced by IFNG than by IFNB. RT1D (class II, green) was only induced by IFNG in OPC, and, to a much less extent, in MO. Nuclei were visualized with DAPI (blue). Scale bar: 50 μm. C: Flow cytometry confirmed that RT1D-positive population of OPC at 48 h after treatment with IFNG was 50% (R1), whereas less than 1% of the population was positive in IFNB-treated cultures. D: Olig2-positive OPC did express RT1D in the presence of IFNG. OPC cultures were treated with IFNG (100 ng/ml) or medium alone for 48 h, and immunolabeled for Olig2 (red) and RT1D (green). Nearly all cells in both OPC cultures were positive for nuclear Olig2, and some Olig2-positive cells were surface positive for RT1D after addition of IFNG (arrowheads). Nuclei were visualized with DAPI (blue). Scale bar: 50 μm.

Article Snippet: Rabbit polyclonal anti-human Olig2 IgG was from Immuno-Biological Laboratories, Co. Ltd. (Takasaki, Japan).

Techniques: Expressing, Immunocytochemistry, Flow Cytometry, Immunolabeling